Journal: Molecular Vision

Article Title: Mertk gene expression and photoreceptor outer segment phagocytosis by cultured rat bone marrow mesenchymal stem cells

doi:

Figure Lengend Snippet: The MER proto-oncogene and the Mertk gene expression ratio between BM-MSCs and RPE cells at different passages and among different passages of BM-MSCs. The first three columns show the ratio of bone marrow mesenchymal stem cells (BM-MSCs) versus RPE cells. The last two columns show the ratio among different passages of BM-MSCs. The asterisk (*) represents p<0.05. The sampling size for each group is 3.The error bars are standard deviation (SD). The data were analyzed with one-way ANOVA. The Mertk gene expression of BM-MSCs was significant greater than RPE cells at P0 and P1.

Article Snippet: Proteins were resolved on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Millipore, MA), which were blocked for 2 h with 5% skim milk and incubated for 24 h with the primary polyclonal antibodies for Mertk (C-term)-Aff-Purified (AP10151PU-N; Acris antibodies, Germany) and β-actin (sc-130657; Santa Cruz Biotechnology, Santa Cruz, CA) at 4 °C, then with horseradish peroxidase-conjugated secondary antibodies (sc-2301; Santa Cruz Biotechnology), and developed with an enhanced chemiluminescent substrate or SuperSignal West Femto Maximum Sensitivity Substrate (Pierce).

Techniques: Gene Expression, Sampling, Standard Deviation

Journal: Molecular Vision

Article Title: Mertk gene expression and photoreceptor outer segment phagocytosis by cultured rat bone marrow mesenchymal stem cells

doi:

Figure Lengend Snippet: Western blot analysis of Mertk protein expression in different passages of RPE cells and BM-MSCs. A : Upper panel, Mertk protein expression. Lower panel, protein samples assayed for β-actin expression, control for protein loading. B : Quantification of Mertk protein expression. The sampling size for each group is 3.The error bars are standard deviation (SD). The data were analyzed with one-way ANOVA. Mertk protein expression in RPE P0 was significantly greater than in RPE P1. Mertk protein expression in MSC P0 was lower than MSC P1. Mertk protein expression in MSC P1 and P2 was significant greater than RPE.

Article Snippet: Proteins were resolved on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Millipore, MA), which were blocked for 2 h with 5% skim milk and incubated for 24 h with the primary polyclonal antibodies for Mertk (C-term)-Aff-Purified (AP10151PU-N; Acris antibodies, Germany) and β-actin (sc-130657; Santa Cruz Biotechnology, Santa Cruz, CA) at 4 °C, then with horseradish peroxidase-conjugated secondary antibodies (sc-2301; Santa Cruz Biotechnology), and developed with an enhanced chemiluminescent substrate or SuperSignal West Femto Maximum Sensitivity Substrate (Pierce).

Techniques: Western Blot, Expressing, Control, Sampling, Standard Deviation